Laboratory diagnosis of methicillin resistance in Staphylococcus aureus: Genotypic or phenotypic methods?
نویسندگان
چکیده
منابع مشابه
Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...
متن کاملComparison of phenotypic versus genotypic methods in the detection of methicillin resistance in Staphylococcus aureus.
BACKGROUND & OBJECTIVE Conventional methods to detect methicillin resistance in Staphylococcus aureus are inadequate as expression of resistance is subject to environmental and conditional expression of PBP2a antigen. The objective of the present study was to determine methicillin resistance in S. aureus by conventional susceptibility (oxacillin disc diffusion and oxacillin MIC) and molecular m...
متن کاملPhenotypic Changes of Oxacillin Resistance in Methicillin-resistant Staphylococcus aureus Strains by Successive Passaging
Background and purpose: Rotational use of antibiotics can decrease antibiotic resistance in Staphylococcus aureus strains. The present study aimed to evaluate the alteration of oxacillin resistance in methicillin-resistant Staphylococcus aureus (MRSA) strain carrying SCCmec III cassette in long-term evolutionary experiments in an antibiotic-free medium. Materials and methods: In a cross-sectio...
متن کاملPhenotypic and Genotypic Characterization of Nosocomial Isolates of Staphylococcus aureus with Reference to Methicillin Resistance
Purpose: To investigate the occurrence, species prevalence, antibacterial resistance, and molecular characteristics of S. aureus isolates from different wards located in a university hospital. Methods: A total of one hundred and fifty S. aureus isolates were recovered from various clinical specimens. The isolates were tested phenotypically by conventional methods and genotypically by polymease ...
متن کاملEvaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)
BACKGROUND Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. METHODS A total of 220 clinical isolates of S. aureus which we...
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ژورنال
عنوان ژورنال: International Journal of Applied and Basic Medical Research
سال: 2014
ISSN: 2229-516X
DOI: 10.4103/2229-516x.125700